lab

Material:
Green plants pigments extract.
Dropper
Cotton ball
Rule
Chloroform
Test tube
Scissors
Pencil
100 mi beaker
Filter paper

Procedure:
Cut a strip of filter paper of 15 x 1 cm. cut one of the ends in a V shape and draw a faint pencil line 1 cm above the pointed end of the paper strip (now called a chromatogram).
Use the dropper to apply a tiny drop of the plant pigments extract on the center of the pencil line. Let the solvent dry and repeat this steps several times until a dark green color can be seen in the paper.
Pour 2 ml of chloroform in the test tube and introduce the paper strip in the tube. A small portion of the tip end must be immersed in the solvent. DO NOT IMMERSE the pigment spot in the solvent.
Place the cotton ball to cover the test tube but DO NOT PUSH the paper with the cotton cap.
Wait 5 - 7 minutes and observe how the solvent travels up the strip.
Remove the chromatogram (paper strip) from the solvent when the solvent nears the top of the test tube.
Measure the distances that the solvent and each of the pigments moved, forming bands of different colors.